ImageJ-Cell Counter

Cell Concentration Counter

CCC is an ImageJ plugin designed to count cells in suspension loaded into a hemocytometer.

Transwell Counter

TC is an ImageJ plugin designed to count membranes of Transwell invasion and migration assays.  TC was developed for high throughput automated counting and manual adjustment.  TC employs a novel algorithm to assist the user in determining the quality of a membrane count and displays this information in a table and frequency plot.  Moreover, the counting algorithm is written within an ImageJ macro and can thus be modified or entirely rewritten by the user.

I encourage any and all script modifications of a noteworthy stature, general suggestions, and/or bug reports to be sent to:

jaobr@my.yorku.ca

Thank you!

ImageJ

Download  and install ImageJ bundled with Java 1.8 or higher.

Plugins

Cell Concentration Calculator
Transwell Counter

Installation

Extract the .zip file within your ImageJ directory.
Done!  The plugin’s files will be placed within the correct directories.

Publication to cite: O’Brien, J., Hayder, H., Peng, C. Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins. J. Vis. Exp. (117), e54719, doi:10.3791/54719 (2016).

Sample Images

Sample Cell Concentration Counter images
Sample Transwell Counter images


Terms and Conditions

Copyright (C) Jacob O’Brien. Permission to use this software and corresponding documentation for educational, research, and not-for-profit purposes, without a fee and without a signed licensing agreement, is granted, subject to the following terms and conditions.

IT IS NOT ALLOWED TO REDISTRIBUTE, SELL, OR LEASE THIS SOFTWARE, OR DERIVATIVE WORKS THEREOF, WITHOUT PERMISSION IN WRITING FROM THE COPYRIGHT HOLDER.

IN NO EVENT SHALL THE COPYRIGHT HOLDER BE LIABLE TO ANY PARTY FOR DIRECT, INDIRECT, SPECIAL, INCIDENTAL, OR CONSEQUENTIAL DAMAGES, OF ANY KIND WHATSOEVER, ARISING OUT OF THE USE OF THIS SOFTWARE AND ITS DOCUMENTATION, EVEN IF ADVISED OF THE POSSIBILITY THEREOF.

THE COPYRIGHT HOLDER SPECIFICALLY DISCLAIMS ANY WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. THE SOFTWARE AND CORRESPONDING DOCUMENTATION IS PROVIDED “AS IS”. THE COPYRIGHT HOLDER HAS NO OBLIGATION TO PROVIDE MAINTENANCE, SUPPORT, UPDATES, ENHANCEMENTS, OR MODIFICATIONS.